By Nathan P. Colowick, Nathan P. Kaplan, Jere P. Segrest, John J. Albers
The severely acclaimed laboratory regular, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this present day - really an important e-book for researchers in all fields of existence sciences
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Additional info for Plasma Lipoproteins Part A: Preparation, Structure, and Molecular Biology
B. ) in the medium with HDL particles even if they are not secreted as such. In principle, such particles could acquire cholesterol by transfer from cell surfaces and thereby participate in cholesterol transport. Discoidal HDL which have been observed in leg lymph of dogs, especially in animals with diet-induced hypercholesterolemia,94 could be synthesized in macrophages of the bone marrow or other peripheral cells. Lipoprotein Processing and Catabolism Chylomicrons (Fig. 3) The acquisition of C and E apolipoproteins by nascent chylomicrons in the interstitial fluid of the intestinal villus and in the blood is critical to 94 C.
Acad. Sci. A. 76, 333 (1979). ~4s D. P. Via, H. A. -L. Cheng, and A. M. , J. Biol. Chem. 260, 7379 (1985). 149T. Mazzone and A. Chait, Arteriosclerosis 2, 487 (1982). 38 OVERVIEW [ 1] some cell membranes. 15° In human plasma, LCAT is largely or entirely associated with a subfraction of HDL that contains apoA-I and apoD, and existing evidence indicates that this fraction is the site of the enzyme's action) 5~The cholesterol substrate for LCAT is derived from the surface of plasma lipoproteins or the plasma membrane of cells.
R. Janero, and D. M. Lane, J. Biol. Chem. 257, 11463 (1982). 22 OVERVIEW  SER Lipids U Sinusoid FIG. 2. General scheme for the assembly and secretion of VLDL from hepatocytes. The nascent particle is thought to originate from the membranes of the smooth endoplasmic reticulum (SER) and to be sequestered within the SER lumen. Apolipoprotein B (and other apolipoproteins) are synthesized on ribosomes of the rough endoplasmic reticulum (RER) and after entry into the RER lumen migrate to its smooth-surfaced terminus, where they associate with the nascent particle.