Download Membrane Fusion Techniques Part B by Nejat Duzgunes (Eds.) PDF

By Nejat Duzgunes (Eds.)

The significantly acclaimed laboratory ordinary, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. due to the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper today--truly a vital e-book for researchers in all fields of lifestyles sciences.

* significant subject matters lined include:
* Cell-cell fusion mediated via viruses and viral proteins
* Conformational adjustments of proteins in the course of membrane fusion
* Membrane fusion in the course of exocytosis
* Intracellular membrane fusion
* Membrane fusion in fertilization
* creation of macromolecules into cells via membrane fusion
* Protoplast fusion

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14, 707 (1989). 39 K. Asano and A. Asano, Biochemistry 27, 1321 (1988). [3] SENDAl VIRUS-INDUCED CELL FUSION 23 HANA Glycoprotein HANA is a glycoprotein with hemagglutination and neuraminidase activities. The cellular virus receptor consists of sialoglycoproteins and sialolipids, and HANA contains both receptor-binding and receptor-degrading activities. The minimal structure recognized by HANA is the sequence NeuAc~2,3Gal? 4~ Five potential acceptor sites for N-linked carbohydrates are detectable in this peptide sequence.

Retroviruses 6, 707 (1990). 4 M. L. Bosch, P. L. Earl, K. Fargnoli, S. Picciafuoco, F. Giombinl, F. Wong-Staal, and G. Franchini, Science 244, 694 (1989). s y. Sakai'and H. Shibuta, J. Virol. 63, 3661 (1989). 6 M. Whitt, P. Zagouras, B. Cruse, and J. K. Rose, J. Virol. 64, 4907 (1990). ~ t © 1993by AcademicPr--~,Inc. METHODSIN ENZYMOLOGY,VOL. 221 Allrightsofrel~'oduclionin any formreserved. [2] VACCINIA IN STUDY OF HIV-MEDIATED MEMBRANE FUSION 13 Recombinant vaccinia virus technology has been used to study membrane fusion mediated by HIV-1 envelope glycoprotein (Env) and its cellular receptor CD4.

188, 112 (1985). 42 y. Hosaka and H. Kitano, Virology 29, 205 (1966). 43 j. Kim, K. Hama, Y. Miyake, and Y. Okada, Virology95, 523 (1979). J. Kim and Y. Okada, J. Membr. Biol. 97, 241 (1987). E~ ~,0 ¢~ ~ ~o~ ~ ~ ~ E~ 24 ~,~ ~ ~ ~o~ ~ - ~-" o. ~ ~,= ~ r ~ '~ ~ ~ ~ ~ ~ . - ~ r~ 25 °~ 26 VIRUS A N D V I R A L P R O T E I N M E D I A T E D C E L L - C E L L FUSION [3] The HL activity of nonaged virions is weak, whereas that of aged ones is strong. This increase in HL activity with aging seems to depend on the formation of large IMPs.

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