Download HPLC of Peptides and Proteins: Methods and Protocols by Marie-Isabel Aguilar PDF

By Marie-Isabel Aguilar

Monash Univ., Clayton, Victoria, Australia. presents entire assurance of all regular HPLC strategies with step by step directions. offers examples of quite a lot of peptide and protein functions and discusses key separation parameters for attaining excessive solution. For researchers. DNLM: Peptides--chemistry.

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Additional info for HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology)

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M. and Wheat, T. E. (1990) Optimisation of high performance liquid chromatographic peptide separations with alternative mobile and stationary phases. J. Chromatogr. 512, 273–281. 22. Poll, D. J. and Harding, D. R. K. (1989) Formic acid as a milder alternative to trifluoroacetic acid and phosphoric acid in two dimensional peptide mapping. J. Chromatogr. 469, 231–239. 22 Aguilar 23. Thevenon, G. and Regnier, F. E. (1989) Reversed-phase liquid chromatography of proteins with strong acids. J. Chromatogr.

Poll, D. J. and Harding, D. R. K. (1989) Formic acid as a milder alternative to trifluoroacetic acid and phosphoric acid in two dimensional peptide mapping. J. Chromatogr. 469, 231–239. 22 Aguilar 23. Thevenon, G. and Regnier, F. E. (1989) Reversed-phase liquid chromatography of proteins with strong acids. J. Chromatogr. 476, 499–511. 24. Mant, C. T. and Hodges, R. S. (1991) The effects of anionic ion-pairing reagents on peptide retention in reversed phase chromatography, in High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation (Mant, C.

Chemicals 1. Ammonium sulfate (NH4)2SO4 2. Milli-Q water. 3. 0. 2. Equipment and Supplies 1. HPLC solvent delivery system with binary gradient capability and ultraviolet (UV) detector. 2. 6 mm ID × 250 mm length, 5 µm particle size, 300Å pore size (See Note 2). 3. Controlled temperature column jacket is recommended. 4. Guard column made of the same packing material as the analytical column is recommended. 5. 45-µm filter. 6. 22 µm porosity. 7. Mobile Phase A: 2 M (NH4)2SO4 in 50 mM buffer (See Note 3).

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