By Marie-Isabel Aguilar
Monash Univ., Clayton, Victoria, Australia. presents entire assurance of all regular HPLC strategies with step by step directions. offers examples of quite a lot of peptide and protein functions and discusses key separation parameters for attaining excessive solution. For researchers. DNLM: Peptides--chemistry.
Read Online or Download HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology) PDF
Similar molecular biology books
Views in Supramolecular Chemistry will relate contemporary advancements and new intriguing techniques in supramolecular chemistry. In supramolecular chemistry, our objective is to appreciate the molecular chemistry past the covalent bond—the sequence will be aware of goal-orientated supramolecular chemistry.
The ebook is a textbook (with many workouts) giving an in-depth account of the sensible use of mathematical modelling within the biomedical sciences. The mathematical point required is usually now not excessive and the emphasis is on what's required to resolve the true organic challenge. the subject material is drawn, e.
The e-book assumes that hodologies (neural circuits, connectome) does generate psyche, i. e. cognitive actual phenomena. additionally, the current textbook additionally silences all of the paintings produced in educational neurobiology outdoor the anglophone culture, attempting to impose this tradition´s narrative over the complete international.
- Advances in Enzymology and Related Areas of Molecular Biology, Volume 59
- Catalytic RNA, Volume 120
- The Molecular Biology of Cancer: A Bridge from Bench to Bedside
- Protein Folding in the Cell
Additional info for HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology)
M. and Wheat, T. E. (1990) Optimisation of high performance liquid chromatographic peptide separations with alternative mobile and stationary phases. J. Chromatogr. 512, 273–281. 22. Poll, D. J. and Harding, D. R. K. (1989) Formic acid as a milder alternative to trifluoroacetic acid and phosphoric acid in two dimensional peptide mapping. J. Chromatogr. 469, 231–239. 22 Aguilar 23. Thevenon, G. and Regnier, F. E. (1989) Reversed-phase liquid chromatography of proteins with strong acids. J. Chromatogr.
Poll, D. J. and Harding, D. R. K. (1989) Formic acid as a milder alternative to trifluoroacetic acid and phosphoric acid in two dimensional peptide mapping. J. Chromatogr. 469, 231–239. 22 Aguilar 23. Thevenon, G. and Regnier, F. E. (1989) Reversed-phase liquid chromatography of proteins with strong acids. J. Chromatogr. 476, 499–511. 24. Mant, C. T. and Hodges, R. S. (1991) The effects of anionic ion-pairing reagents on peptide retention in reversed phase chromatography, in High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation (Mant, C.
Chemicals 1. Ammonium sulfate (NH4)2SO4 2. Milli-Q water. 3. 0. 2. Equipment and Supplies 1. HPLC solvent delivery system with binary gradient capability and ultraviolet (UV) detector. 2. 6 mm ID × 250 mm length, 5 µm particle size, 300Å pore size (See Note 2). 3. Controlled temperature column jacket is recommended. 4. Guard column made of the same packing material as the analytical column is recommended. 5. 45-µm filter. 6. 22 µm porosity. 7. Mobile Phase A: 2 M (NH4)2SO4 in 50 mM buffer (See Note 3).