By Donald C. Chang, Bruce M. Chassy, James Saunders MD, Arthur E. Sowers
Electroporation is a good technique to introduce macromolecules resembling DNA right into a big range of cells. Electrofusion leads to the fusion of cells and will be used to supply genetic hybrids or hybridoma cells. consultant to Electroporation and Electrofusion is designed to serve the wishes of scholars, skilled researchers, and novices to the sector. it's a entire handbook that provides, in a single source, updated, easy-to-follow protocols worthwhile for effective electroporation and electrofusion of micro organism, yeast, and plant and animal cells, in addition to history informati. Read more...
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Additional resources for Guide to Electroporation and Electrofusion
Positive electrode was to the right of the cell and negative electrode to the left. Intensity profiles are shown at the bottom. 34 Kazuhiko Kinosita, J r . et al. indicates that the cell m e m b r a n e can withstand such a high ΔΨ at least for tens of microseconds. 75 V, the cell behaved as predicted in Eq. (1), or as a spherical conductor surrounded by a thin dielectric membrane of negligible conductance. III. Electroporation Revealed in the Behavior of Transmembrane Potential A. S a t u r a t i o n of T r a n s m e m b r a n e P o t e n t i a l Under an external electric field for which the theoretical A ^ m ax is greater than about 1 V, the behavior of fluorescence response was quite different from that in Fig.
Introduction II. Visualization of Transmembrane Potential III. Electroporation Revealed in the Behavior of Transmembrane Potential A. Saturation of Transmembrane Potential B. Estimation of M e m b r a n e Conductance C. Kinetics of Electroporation D. Recovery after Poration E. Molecular Orientation in Porated Membranes IV. Large-Hole Formation and Deformation in Giant Liposomes A. Large-Hole Formation B. Deformation under Intense Electric Fields V. Permeation across Porated Membranes A. Calcium Influx into Porated Eggs B.
Naturforsch. 34a, 1063-1065. Helfrich, W. (1974). Deformation of lipid bilayer spheres by electric fields. Z. Naturforsch. 29c, 182-183. , Itoh, H . , Jr. (1991). Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane conductance. Biophys. J. 59, 209—220. , and Wakabayashi, N . (1991a). Deformation of vesicles under the influence of strong electric fields. Jpn. J. Appl. , 30, 1141-1148. Hyuga H . , and Wakabayashi, N . (1991b). J. Appl. , 30, 1333-1335.