By Yujun George Zheng
Epigenetic Technological Applications is a compilation of state of the art applied sciences occupied with epigenetic examine. Epigenetics is an exhilarating new box of biology examine, and plenty of applied sciences are invented and built particularly for epigenetics examine. With chapters masking the most recent advancements in crystallography, computational modeling, the makes use of of histones, and extra, Epigenetic Technological Applications addresses the query of ways those new rules, approaches, and options may be utilized to present epigenetics learn, and the way they could hold pushing discovery ahead and past the epigenetic realm.
- Discusses applied sciences which are severe for epigenetic study and application
- Includes epigenetic purposes for cutting-edge technologies
- Contains a world point of view at the way forward for epigenetics
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Additional resources for Epigenetic Technological Applications
5% of the genome) that were then extracted by a standard size selection method. Four years after this foundational RRBS study, a greatly improved RRBS method was proposed that replaced the BglII restriction enzyme with MspI and also reduced the size selection to 40À220 bp . Since the core sequence of the MspI restriction cut site, CΔCGG, is a CpG dinucleotide, utilizing this enzyme in the RRBS method would ensure that every sequence read will contain at least one unmethylated or methylated CpG.
25] Terragni J, Bitinaite J, Zheng Y, Pradhan S. Biochemical characterization of recombinant betaglucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes. Biochemistry 2012;51(5):1009À19.  Szwagierczak A, Bultmann S, Schmidt CS, Spada F, Leonhardt H. Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucleic Acids Res 2010;38(19):e181.  Waalwijk C, Flavell RA. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
The OxBS-Seq method was first detailed in a study by Booth et al. around the same time the competing TAB-Seq method was first proposed [64,65]. While the TAB-Seq technique differentiates 5mC from 5hmC by selectively oxidizing the 5mC before bisulfite conversion, the OxBS-Seq technique makes this differentiation by selectively oxidizing the 5hmC. Like TAB-Seq the OxBS-Seq method is done in conjunction with standard BS-Seq to allow for both the identification of 5mC and 5hmC throughout the genome.