Download Cryo-EM Part A: Sample Preparation and Data Collection by Grant Jensen PDF

By Grant Jensen

This quantity is devoted to an outline of the tools, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the guidelines, intrumentation, and techniques underlying all cryo-EM methods which enable practictioners to simply flow among them. inside of every one part, the articles are ordered in keeping with the commonest symmetry of the pattern to which their tools are applied.* Includes time-tested core methods and new innovations applicable to any researcher * equipment incorporated are worthy to either established researchers and newbies to the sector * correct history and reference details given for techniques can be utilized as a consultant

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I extracted the phases and amplitudes along the layer lines and calculated our second 3D map of a single phage tail. The first map of the particle, which we had done with optically measured phases, was somewhat different from the second, all digital maps presumably due to differences in the phases. The bigger features were similar but the finer details were changed. The computer-generated phases were more rigorously obtained and we believed them, but we needed to look at 3D maps generated from images of other T4 tail particles.

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