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By Nader Hassanzadeh

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The most widely used transposon in Gram-negative bacteria is Tn5, which transposes at high frequency, has relatively little target sequence specificity, and does not share homology with genomic sequences of most bacterial species. However, transposon mutagenesis can have the following technical limitations: The transposase gene must be expressed in the target host, The transposon must be introduced into the host on a suicide vector The transposase gene on the transposon can be expressed in subsequent generations, which may result in genetic instability.

The original molecule is irreparably damaged as a result. At the same time, a staggered cut is introduced into a target site into which the element is ligated. This mechanism results in the duplication of the target site sequence on each side of the inserted element. Martin E. Mulligan, 1996-2002 61 Replicative transposition     It is a mechanism whereby the element replicates itself from place to place. This mechanism requires staggered cuts on either side of the mobile element and in the target site.

38 4. Transposable elements   Transposons Insertion sequences (IS) Transposable elements have become valuable mutagenic tools for genetic and molecular analysis in many different bacteria. 39 Transposable genetic elements in Bacteria    1. 2. 3. 4. Mobile genetic elements were discovered in bacteria in the late 1960s. They were also recognized because they caused a particular type of mutation which was unstable. We can recognize four types of bacterial transposon: Insertion Sequences Transposons Composite Transposons Bacteriophage Elements Martin E.

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